Deposition Serial
The diagnosis is confirmed by the presence of E. histolytica in feces or tissues.
be examined for motile trophozoites, prepared fresh liquid, semi-formed stool recently. (Serial stool)
Amoebae usually more easily shown in the flakes of mucus bloody stool.
"Sometimes examines stool samples 3-6 to reach the diagnosis, preserving all the tests, where to defer the exam, to preserve the trophozoites and cysts."
In symptomatic patients, proctoscopy can show mucosal lesions. You have to make an aspiration of injuries and an examination of the aspirate in search of trophozoites.
Diagnosis of extraintestinal amebiasis is more difficult. In general, stool examination is negative and rarely can be demonstrated the presence of trophozoites in the pus. In some cases with clinical suspicion of amoebic liver abscess the only method of diagnosis that has any use is the administration of amebicides test.
serological tests show positive results in almost all patients with amebic liver abscess, and in more than 80% of those with acute amoebic dysentery.
ELISA
immunosorbent assays (EIA) and methods of analysis laboratory called Enzyme-linked immunosorbent assay (ELISA) are available as a diagnostic tool to aid in the detection of Entamoeba histolytica and should be used in addition microscopic study, particularly the tests reveal the presence of amebic antigen and are commonly used in clinical laboratories to detect the presence of these antigens in their feces demonstrating its high sensitivity and high specificity that can be used by technologists to detect its presence.
be examined for motile trophozoites, prepared fresh liquid, semi-formed stool recently. (Serial stool)
Amoebae usually more easily shown in the flakes of mucus bloody stool.
"Sometimes examines stool samples 3-6 to reach the diagnosis, preserving all the tests, where to defer the exam, to preserve the trophozoites and cysts."
In symptomatic patients, proctoscopy can show mucosal lesions. You have to make an aspiration of injuries and an examination of the aspirate in search of trophozoites.
Diagnosis of extraintestinal amebiasis is more difficult. In general, stool examination is negative and rarely can be demonstrated the presence of trophozoites in the pus. In some cases with clinical suspicion of amoebic liver abscess the only method of diagnosis that has any use is the administration of amebicides test.
serological tests show positive results in almost all patients with amebic liver abscess, and in more than 80% of those with acute amoebic dysentery.
ELISA
immunosorbent assays (EIA) and methods of analysis laboratory called Enzyme-linked immunosorbent assay (ELISA) are available as a diagnostic tool to aid in the detection of Entamoeba histolytica and should be used in addition microscopic study, particularly the tests reveal the presence of amebic antigen and are commonly used in clinical laboratories to detect the presence of these antigens in their feces demonstrating its high sensitivity and high specificity that can be used by technologists to detect its presence.
Moreover, the detection of antibodies in the serum has been considered it crucial to study extra-intestinal infections such as amebic liver abscess. The sensitivity of antibody testing of Entamoeba histolytica in a study diagnosis of amebic liver abscess has been reported that almost 100%.
Reaction Polymerase Chain
As the analysis of antigen detection, Chain Reaction (PCR) has also been considered as a diagnostic tool. PCR techniques have several advantages and disadvantages. For example, PCR methods are highly specific and sensitive. Chain Reaction Polymerase is able to detect a difference trophozoites per sample to ELISA techniques which are not able to detect low levels of amoebic antigens.
PCR methods are considered very specific, which are aimed at detecting specific DNA of the organism sought. The disadvantages of using PCR is influenced by the fact that require a high level of technical skills laboratory, are extremely laborious and expensive performance compared to ELISA techniques is very complex.
Moreover, the problem of contamination fecal techniques DNA amplification and detection of amebic is very common.
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